Cell Direct RT qPCR kit Operation video

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Cell Direct RT qPCR kit Operation video

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April 02, 2022

Connexion De Catégorie: kit de qPCR

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Protocol of Foregene Cell Direct RT-qPCR kit
-7min get RNA template;
-RT and qPCR in one tube
-Fast and high efficiency,high specificity

Balises:

#99 #9% un ACP de l'étape droite #un kit 1.7ml×3 de qPCR d'étape

Brief: Discover the Buffer CL One Step RT QPCR Kit, designed for quick and easy RNA release from cultured cells. This kit eliminates RNA purification, delivering results in just 7 minutes. Ideal for gene expression analysis, siRNA verification, and drug screening.

Related Product Features:

Simple and effective Cell Direct RT technology for RNA extraction in 7 minutes.
Requires as few as 10 cells for testing, minimizing sample demand.
Compatibilité à haut débit avec les plaques de 384, 96, 24, 12 et 6 puits.
Includes DNA Eraser to remove genomes and reduce experimental interference.
Optimized RT and qPCR system for efficient reverse transcription and specific PCR.
Strong inhibitor tolerance for direct use of lysate in RT-qPCR.
Contains Foregene reverse transcriptase and Hot D-Taq DNA polymerase.
Stable storage options with components kept at 4°C or -20°C.

FAQ:

What is the main advantage of the Buffer CL One Step RT QPCR Kit?

The kit eliminates the need for RNA purification, allowing RNA extraction and RT-qPCR in just 7 minutes, saving time and labor.
How many cells are required for testing with this kit?

The kit requires as few as 10 cells, making it suitable for low-sample applications.
What applications is this kit suitable for?

This kit is ideal for gene expression analysis, siRNA-mediated gene silencing verification, and drug screening in cultured cells.
How should the kit components be stored?

Part I should be stored at 4°C, Part II at -20°C, and Foregene Protease Plus II at 4°C. The 2×Direct qPCR Mix-SYBR should be kept at -20°C in the dark.